Modulation and elimination of yeast prions by protein chaperones and co-chaperones.

Molecular Chaperones and Co-chaperones as Therapeutic Targets for Cancer

Cancer is a devastating disease that has affected millions of people, consumed tremendous efforts in the treatment/care and is incurable. Statistics from www.seer.cancer.gov show 14,140,254 people were living with cancer in 2013 in US with 1,685,210 new cases projected and 595,690 estimated deaths to occur due to cancer in 2016. Number of people surviving cancer (measured as 5-year survival rat...

Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions.

Saccharomyces cerevisiae Hsp104 and Escherichia coli ClpB are Hsp100 family AAA+ chaperones that provide stress tolerance by cooperating with Hsp70 and Hsp40 to solubilize aggregated protein. Hsp104 also remodels amyloid in vitro and promotes propagation of amyloid prions in yeast, but ClpB does neither, leading to a view that Hsp104 evolved these activities. Although biochemical analyses ident...

Interactions between Endogenous Prions, Chaperones and Poly-glutamine Proteins in the Yeast Model

Prion-Associated Toxicity is Rescued by Elimination of Cotranslational Chaperones

The nascent polypeptide-associated complex (NAC) is a highly conserved but poorly characterized triad of proteins that bind near the ribosome exit tunnel. The NAC is the first cotranslational factor to bind to polypeptides and assist with their proper folding. Surprisingly, we found that deletion of NAC subunits in Saccharomyces cerevisiae rescues toxicity associated with the strong [PSI+] prio...

BiP Co-chaperones and ER Calcium Leakage

Stefan Schorr, Marie-Christine Klein, Igor Gamayun, Armin Melnyk, Martin Jung, Nico Schäuble, Qian Wang, Birgit Hemmis, Florian Bochen, Markus Greiner, Pavel Lampel, Sabine Katharina Urban, Sarah Haßdenteufel, Johanna Dudek, Xing-Zhen Chen, Richard Wagner, Adolfo Cavalié, and Richard Zimmermann Medical Biochemistry and Molecular Biology, Saarland University, 66421 Homburg, Germany; Department o...